ABSTRACT
Aim To investigate the inhibitory effect of copper gluconate and disulfiran on the PLpro of SARS-CoV-2 and to explore the effect of combination of them on PLpro.Methods SARS-CoV-2 PLpro was purified by recombinant expression technology of he effects of copper gluconate and disulfiram on PLpro activity were studied by enzyme kinetic method.Results Copper gluconate and disulfiram had high inhibitory activity on SARS-CoV-2 PLpro,Ubiquitin-7-Amino-4-methylcoumarin(Ub-AMC)was used as the fluorescent substrate.The IC50 of copper gluconate was 33.02 nmol.L-1,showing competitive inhibition,and the IC50 of disulfiram was 480.4 nmol.L-1,showing non competitive inhibition,and the combination of the two inhibitors showed the advantage of synergistic inhibition.Conclusions Copper gluconate and disulfiram have a high inhibitory effect on SARS-CoV-2 PLpro protein,and the combination shows the advantage of synergistic inhibition. Copyright © 2022 Publication Centre of Anhui Medical University. All rights reserved.
ABSTRACT
It is over 70 years since two orthopedic surgeons invented the Milwaukee brace for the treatment of children with scoliosis. Since then, clinicians and researchers have been inspired to design ever more effective braces to correct 3-D spinal deformities. This book presents papers from the bi-annual meeting of the International Research Society of Spinal Deformities (IRSSD), held as a virtual event on 22 and 23 January 2021. The IRSSD concentrates on research into spinal deformity with clinical applications. In addition to 3D assessment of the spine, researchers also explore spinal biomechanics, etiopathogenesis, and innovative conservative and surgical therapies with the goal of integrating science with clinical care to improve patient care. The 2021 meeting was originally scheduled to take place in Milwaukee, Wisconsin, USA, but was instead held in a virtual format due to the Covid 19 pandemic. Despite this change, the meeting still allowed valuable interaction and open discussion among practitioners from around the world, and keynote speakers and authors contributed the 44 short papers and 47 abstracts included here. The papers are grouped under 17 chapter headings, and cover a wide range of topics, including biologic and biomechanical benchmarks, clinical evaluation, conservative treatments and surgical approaches. Diagnostic assessments and non-surgical treatments of EOS are also emphasized and elucidated. The book will be of interest to all those whose work is related to the treatment and care of patients with spinal deformities. © 2021 The authors and IOS Press.